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Promega random hexanucleotide primers
Random Hexanucleotide Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexanucleotide+primers/random+hexanucleotide+primers/pm38030018-110-33-35
Average 90 stars, based on 1 article reviews
random hexanucleotide primers - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Complete Genomic Sequence of an Australian Sindbis Virus Isolated 44 Years Ago Reveals Unique Indels in the E2 and nsP3 Proteins
Article Snippet: .. Viral RNA was extracted from 140 μl of this tissue culture supernatant using the QIAamp viral RNA minikit (Qiagen, USA) and reverse transcribed using random hexanucleotide primers (Promega, USA) and SuperScript III reverse transcriptase (Invitrogen, USA), according to the manufacturer’s instructions. .. Overlapping fragments of 1.0 to 1.5 kb of the resultant cDNA were amplified by using Expand long template DNA polymerase (Roche) and virus-specific primers corresponding to the sequence of SINV strain MRE-16 (GenBank accession numbers AF492770 and U90536 ).

Article Title: Complete Genomic Sequence of an Australian Sindbis Virus Isolated 44 Years Ago Reveals Unique Indels in the E2 and nsP3 Proteins
Article Snippet: .. Viral RNA was extracted from 140 l of this tissue culture supernatant using the QIAamp viral RNA minikit (Qiagen, USA) and reverse transcribed using random hexanucleotide primers (Promega, USA) and SuperScript III reverse transcriptase (Invitrogen, USA), according to the manufacturer’s instructions. .. Overlapping fragments of 1.0 to 1.5 kb of the resultant cDNA were amplified by using Expand long template DNA polymerase (Roche) and virus-specific primers corresponding to the sequence of SINV strain MRE-16 (GenBank accession numbers AF492770 and U90536).

Article Title: Mosquito and Arbovirus Surveillance at Bradshaw Field Training Area, Northern Territory, Australia.
Article Snippet: Mosquito homogenates were extracted for viral RNA by using the QIAamp Viral RNA Mini kit (Qiagen, Hiden, Germany) according to manufacturer instructions. .. The extracted mosquito RNA was reverse transcribed into cDNA by using random hexanucleotide primers (Promega, Madison, WI) and Superscript III Reverse Transcriptase (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. ..

Article Title: Transcriptome analysis of the mobile genome ICE clc in Pseudomonas knackmussii B13
Article Snippet: .. Briefly, each 50-μL reaction contained 10 μg of total RNA, 1.25 μg of random hexanucleotide primers (Promega), 100 μM each of unlabeled dATP, dGTP, and dTTP (Invitrogen), 25 μM of unlabeled dCTP (Invitrogen), 25 μM of cyanine-3-labeled dCTP (Perkin-Elmer), 25 U SUPERase•In (Ambion), and 400 U Superscript II reverse transcriptase (Invitrogen). ..

Article Title: Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies
Article Snippet: .. Reverse transcription of total RNA was performed at 37 °C using the Moloney murine leukemia virus reverse transcriptase (Invitrogen, Carlsbad, CA) and random hexanucleotide primers (Promega, Madison, WI). .. Real-time quantitative PCR analyses were performed on a Light Cycler 480 SYBR Green I master and a Light Cycler 480 apparatus (both from Roche Diagnostics, Indianapolis, IN).

Article Title: Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan - The pacific oyster (Crassostrea gigas).
Article Snippet: Recovered RNA was then further purified on Nucleospin RNAII columns (Macherey-Nagel). .. After treatment for 20 min at 37 ◦C with 1 U of DNase I (Sigma) to prevent genomic DNA contamination, 1 μg of total RNA was reverse transcribed using 1 μg of random hexanucleotide primers (Promega), 0.5 mM dNTPs and 200 U MMuLV Reverse Transcriptase (Promega) at 37 ◦C for 1 h in the appropriate buffer. ..

Article Title: Cathepsin-D, a Key Protease in Breast Cancer, Is Up-Regulated in Obese Mouse and Human Adipose Tissue, and Controls Adipogenesis
Article Snippet: Total RNA was extracted using the Reasy minikit (QIAGEN Sciences, Maryland) according to the manufacturer's instructions. .. Reverse transcription of total RNA was performed at 37°C using Moloney murine leukemia virus reverse transcriptase enzyme (Invitrogen, Carlsbad, CA) and random hexanucleotide primers (Promega, Madison, WI). .. Quantitative PCR was carried out by real-time PCR using a LightCycler and the DNA double-strand-specific SYBR green I dye for detection (Roche, Basel, Switzerland).

other:

Article Title: Anti-cathepsin D immunotherapy triggers both innate and adaptive anti-tumour immunity in breast cancer.
Article Snippet: Funding information French National Research Agency (ANR); University of Montpellier; Inserm Transfert, Région Occitanie; Ligue Régionale de l'Herault; Ligue Régionale de la Charente Maritime; Ligue Nationale contre le Cancer Abstract Background and Purpose: Triple-negative breast cancer (TNBC) has poorer outcomes than other breast cancers (BC), including HER2 BC.. Cathepsin D (CathD) is a poor prognosis marker overproduced by BC cells, hypersecreted in the tumour microenvironment with tumour-promoting activity.. Here, we characterized the immunomodulatory activity of the anti-CathD antibody F1 and its improved Fabaglycosylated version (F1M1) in immunocompetent mouse models of TNBC (C57BL/6 mice harbouring E0771 cell grafts) and HER2-amplified BC (BALB/c mice harbouring TUBO cell grafts).

Virus:

Article Title: Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies
Article Snippet: .. Reverse transcription of total RNA was performed at 37 °C using the Moloney murine leukemia virus reverse transcriptase (Invitrogen, Carlsbad, CA) and random hexanucleotide primers (Promega, Madison, WI). .. Real-time quantitative PCR analyses were performed on a Light Cycler 480 SYBR Green I master and a Light Cycler 480 apparatus (both from Roche Diagnostics, Indianapolis, IN).

Article Title: Cathepsin-D, a Key Protease in Breast Cancer, Is Up-Regulated in Obese Mouse and Human Adipose Tissue, and Controls Adipogenesis
Article Snippet: Total RNA was extracted using the Reasy minikit (QIAGEN Sciences, Maryland) according to the manufacturer's instructions. .. Reverse transcription of total RNA was performed at 37°C using Moloney murine leukemia virus reverse transcriptase enzyme (Invitrogen, Carlsbad, CA) and random hexanucleotide primers (Promega, Madison, WI). .. Quantitative PCR was carried out by real-time PCR using a LightCycler and the DNA double-strand-specific SYBR green I dye for detection (Roche, Basel, Switzerland).



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